Open Access Methodology article

A qRT-PCR assay for the expression of all Mal d 1 isoallergen genes

Giulia Pagliarani12*, Roberta Paris24, Paul Arens1, Stefano Tartarini2, Giampaolo Ricci3, Marinus JM Smulders1 and W Eric van de Weg1

Author Affiliations

1 Wageningen UR Plant Breeding, Plant Research International, Droevendaalsesteeg 1, Wageningen, PB, 6708, The Netherlands

2 Department of Fruit Tree and Woody Plant Sciences, University of Bologna, Viale Fanin 46, Bologna, 40127, Italy

3 Department of Paediatrics, University of Bologna, Via Massarenti 11, Bologna, 40138, Italy

4 Present address: Consiglio per la Ricerca e la sperimentazione in Agricoltura-Centro di Ricerca per le Colture Industriali, via di Corticella 133, Bologna, 40128, Italy

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BMC Plant Biology 2013, 13:51  doi:10.1186/1471-2229-13-51

Published: 23 March 2013

Additional files

Additional file 1:

List of Mal d 1 sequences included in the alignment.

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Additional file 2:

Alignment of the 31 coding sequences of the Mal d 1 isoallergen genes. Alignment of the 31 coding sequences of the Mal d 1 isoallergen genes retrieved from the ‘Golden Delicious’ genome sequence. The alignment was performed using MegAlign (DNASTAR Lasergene v8.0). Each sequence was reported using the name of the related gene and the accession number from the Apple GBrowse - Malus x domestica v1.0 [22]. The mismatched residues in the consensus sequence are highlighted in white.

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Additional file 3:

Results of direct sequencing. The results of the BLASTN analysis of the sequences retrieved from the direct sequencing of Mal d 1 amplicons from 10 different apple cultivars. The table lists the targeted gene, the name of the primer pair and the ID of the most similar sequence in the database or in the Gbrowse of the apple genome for each of the cultivars [22]; the presence/absence of the SNPs in these reference sequences is also indicated.

Format: XLS Size: 41KB Download file

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