Complementation analysis of GmPHT1 genes in the yeast double mutant PAM2. Wild type (WT) and mutant (PAM2) yeast strains containing either an empty pYES-DEST52 vector (Empty vector) or pYES-DEST52 (bearing one of the GmPHT1 sequences). Serial dilutions were spotted onto a selective medium supplemented with low concentrations (10 μM) of Pi, with either glucose (Glu, non-induced medium) or galactose (Gal, induced medium) as the carbon source. Each spot represented 5 μl yeast culture, diluted from a master culture, as indicated. Yellow color indicates the cell in the spots grew well. Images were captured after three days.
Fan et al. BMC Plant Biology 2013 13:48 doi:10.1186/1471-2229-13-48