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Open Access Research article

The pattern of Phosphate transporter 1 genes evolutionary divergence in Glycine max L.

Chengming Fan1, Xu Wang1, Ruibo Hu2, Yahui Wang3, Chaowen Xiao1, Ying Jiang1, Xiaomei Zhang1, Changying Zheng3 and Yong-Fu Fu1*

Author Affiliations

1 MOA Key Lab of Soybean Biology (Beijing), National K’ey Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Haidian District, Beijing, 100081, China

2 CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and BioProcess Technology, Chinese Academy of Sciences, Qingdao, Shandong, 266101, China

3 College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, China

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BMC Plant Biology 2013, 13:48  doi:10.1186/1471-2229-13-48

Published: 20 March 2013

Additional files

Additional file 1:

A list of known plant PHT1 genes.

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Additional file 2:

The nomenclature of soybean PHT1s in different papers.

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Additional file 3:

GmPHT1 promoters, genes and gene structures.

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Additional file 4:

PHT1 phylogenetic tree.

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Additional file 5:

A, The cis-acting regulatory DNA elements present within the 14GmPHT1promoters. B, The PHT1 gene family in M.truncatula and synteny blocks harbouring PHT1s among soybean and Medicago. C, Synteny between GmPHT1 containing segments in soybean. D, Relative transcript abundance of the various GmPHT1 genes across different tissues. E, The information of the set of GmPHT1 genes and the primer sequences used for RT-qPCR and gene cloning experiments. F, The Ks and Ka values among GmPHT1 genes.

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Additional file 6:

The growth of the yeast double mutant PAM2 in the presence of various concentrations of Pi.

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Additional file 7:

Transient transcription of the GmPHT1;1-YFP, GmPHT1;5-YFP and GmPHT1;6-YFP fusions in plasmolyzed onion epidermal cells.

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Additional file 8:

Motifs and domains shared by the GmPHT1 and other PHT1 proteins.

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Additional file 9:

The appearance of soybean roots in plants subjected to different Pi concentration conditions.

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