Additional file 3.

Validation of the microarray data by RT-PCR assays on genes that exhibited AZ preferential expression by the microarray assays. Transcript levels of 10 genes in the pedicel regions, AZ, Prox and Dis, were compared for the microarray and RT-PCR assays. The results obtained by the two methods showed good consistency for all the examined genes. As an internal control (Ctrl) for the RT-PCR assays, SAND (SGN-U316474) was used [96].

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Nakano et al. BMC Plant Biology 2013 13:40   doi:10.1186/1471-2229-13-40