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Open Access Highly Accessed Research article

Selaginella moellendorffii has a reduced and highly conserved expansin superfamily with genes more closely related to angiosperms than to bryophytes

Robert E Carey12*, Nathan K Hepler3 and Daniel J Cosgrove2

Author affiliations

1 Department of Biology, Lebanon Valley College, 101 N. College Ave., Annville, PA, 17003, USA

2 Department of Biology, The Pennsylvania State University, 208 Mueller Lab, University Park, PA, 16802, USA

3 Program in Biochemistry and Molecular Biology, Lebanon Valley College, 101 N. College Ave., Annville, PA, 17003, USA

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Citation and License

BMC Plant Biology 2013, 13:4  doi:10.1186/1471-2229-13-4

Published: 3 January 2013

Abstract

Background

Expansins are plant cell wall loosening proteins encoded by a large superfamily of genes, consisting of four families named EXPA, EXPB, EXLA, and EXLB. The evolution of the expansin superfamily is well understood in angiosperms, thanks to synteny-based evolutionary studies of the gene superfamily in Arabidopsis, rice, and Populus. Analysis of the expansin superfamily in the moss Physcomitrella patens revealed a superfamily without EXLA or EXLB genes that has evolved considerably and independently of angiosperm expansins. The sequencing of the Selaginella moellendorffii genome has allowed us to extend these analyses into an early diverging vascular plant.

Results

The expansin superfamily in Selaginella moellendorffii has now been assembled from genomic scaffolds. A smaller (and less diverse) superfamily is revealed, consistent with studies of other gene families in Selaginella. Selaginella has an expansin superfamily, which, like Physcomitrella, lacks EXLA or EXLB genes, but does contain two EXPA genes that are related to a particular Arabidopsis-rice clade involved in root hair development.

Conclusions

From sequence-based phylogenetic analysis, most Selaginella expansins lie outside the Arabidopsis-rice clades, leading us to estimate the minimum number of expansins present in the last common ancestor of Selaginella and angiosperms at 2 EXPA genes and 1 EXPB gene. These results confirm Selaginella as an important intermediary between bryophytes and angiosperms.

Keywords:
Expansin; Selaginella moellendorffii; Cell wall loosening; Gene family evolution; Plant phylogenetics