Western blot analysis of Arabidopsis Twinkle homologue expression. A. Lane 1, Molecular weight markers, Lane 2, leaf tissue from 6-week plants; lane 3, shoot apex tissue; lane 4, total plant tissue protein; lane 5, cotyledon protein. The panel on the left was incubated with antibody against the Twinkle protein. The panel on the right was incubated with histone H3 antibody as a loading control. B. Relative levels of Twinkle protein relative to a nuclear tubulin protein control in Arabidopsis seedlings harvested at the times indicated. The average of three independent western blots is shown for each time point (weeks 1–5 and 10). Error bars indicate the SEM (standard error of the mean).
Diray-Arce et al. BMC Plant Biology 2013 13:36 doi:10.1186/1471-2229-13-36