Figure 6.

Histochemical detection of lignin, phenolics, and flavonoids. Leaves from plants treated with water (B, E, G) or 30 mM thiamine (A, C, D, F, H, I) were collected at 1 dpt, and used for microscopic studies. (A, B, C) Leaves from plants were used for subcellular detection of lignin accumulation as a red coloration under light microscope following phloroglucinol-HCl staining. (D, E, F) Leaf segments were examined under auto-fluorescence (UV light Filter); white–blue coloration indicates the presence of phenolics. (G, H, I) Leaf sections (10 μm of thickness) were stained with Wilson’s reagent and observed under UV light; yellow coloration indicates presence of flavonoids. Six leaves from different plants were assessed for each treatment and the whole experiment was repeated three times with similar results. Bars = 40 μM for (A, B, D, E, G, H) and 20 μm for (C, F, I).

Boubakri et al. BMC Plant Biology 2013 13:31   doi:10.1186/1471-2229-13-31
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