Time course of transcript accumulation of genes of the phenylpropanoid pathway after thiamine treatment. Grapevine plants (cv. Chardonnay) were treated with 30 mM thiamine (solid bars) or water (white bars) and leaves were collected at different time intervals post-treatment (12, 24, 36, 48, and 72 hpt). Gene expression was analyzed by real-time q-PCR. Genes of interest are: (A) PAL (phenylalanine ammonialyase), (B) C4H1 (cinnamate 4-hydroxylase), (C) 4CL1 (4-coumarate:coenzyme A ligase), (D) CCR2 (cinnamoyl:coenzyme A reductase), (E) CHS1 (chalcone synthase) and (F) STS1 (stilbene synthase1). Results are presented as normalized fold-expression of defense genes in thiamine-treated samples compared to expression in water-treated controls at the same time point, which is set to 1. Results are mean ± standard deviations of 3 independent experiments.
Boubakri et al. BMC Plant Biology 2013 13:31 doi:10.1186/1471-2229-13-31