The PpGT47A gene does not rescue the monosaccharide composition of Arabidopsis irx10 irx10-L mutant stem tissue.A) Monosaccharide composition of stems material isolated from mature Arabidopsis wild-type plants, young wild-type plants with a 30mm stem, irx10 irx10-L double mutants and 3 independent lines of irx10 irx10-L double mutants complemented by the Physcomitrella IRX10 homolog (AtPpGT47A:16, AtPpGT47A:8, AtPpGT47A:5). Data from complemented plants are based on pooled stem material in three technical replicates for each of 3 independent biological samples pooled from multiple plants (except for samples indicated by * where only a single pooled sample was used). Data is displayed as molecular percentage of measured sugars obtained by TMS analysis from 500 μg of amylase treated AIR starting material for each sample. Error bars represent standard deviation based on three replicates.
Hörnblad et al. BMC Plant Biology 2013 13:3 doi:10.1186/1471-2229-13-3