Expression of TvQR1 and TvPirin under haustorium-inducing and -non-inducing conditions. (A) Toxicity effects of HIFs and non-HIFs on T. versicolor roots and haustorium formation. D20: 20 μM DMBQ, D100: 100 μM DMBQ, J50: 50 μM juglone, P1000: 1 mM peonidin. (B) Northern blot analysis of the spatial and temporal expression of TvQR1 and TvPirin following treatment with HIFs and non-HIFs. 0: no treatment, H: water, E: 0.1% ethanol (solvent of peonidin), D: 20 μM DMBQ, J: 10 μM juglone, P: 30 μM peonidin. TvRuvB DNA helicase is a constitutively expressed gene. (C) Differential expression of TvQR1 and TvPirin under different HIFs and non-HIFs measured by qRT-PCR. H: water, J: 10 μM juglone, D: 20 μM DMBQ, P: 30 μM peonidin.
Ngo et al. BMC Plant Biology 2013 13:28 doi:10.1186/1471-2229-13-28