Hormone-induced callus formation is not affected in ucn-1 seedlings and ucn-1 protrusions do not accumulate detectable DR5rev::GFP and ARR5::GUS reporter signals. (A-D) Confocal micrographs of live wild-type (Ler) and ucn-1 plants carrying the DR5rev::GFP reporter. Overlays of GFP and bright-field channels. No difference in signal detected between wild-type and ucn-1. (A, B) Stage 2-III ovules. (C, D) Stage 3-III ovules. Note the absence of detectable signal in the ucn-1 protrusions (arrows). (E-H) Plants carrying the ARR5::GUS reporter. No difference in signal detected between wild-type and ucn-1. (E, F) Light micrographs of fixed stage 14 flowers (4 h incubation in GUS staining solution). Arrowheads indicate signal at the junction between filament and anther. (G, H) Differential interference contrast micrographs of fixed stage 3-IV ovules (16 h incubation in GUS staining solution). Note the absence of signal in the ucn-1 protrusion (outlined). (I) Light micrograph of a 30-days-old callus grown from germinated wild-type (Ler) seedling grown on callus induction medium (CIM). (J) Light micrograph of a 30-days-old callus grown from germinated ucn-1 seedling grown on CIM. Callus is of comparable size than the one in (I). Scale bars: (A-D): 20 μm, (G, H) 30 μm, (E, F, I, J) 0.5 mm.
Enugutti and Schneitz BMC Plant Biology 2013 13:2 doi:10.1186/1471-2229-13-2