Additional file 3.

The PRC2 regulates carotenoid production in A. coerulea petals. A. High magnification views of epidermal cells in A. x coerulea petal limbs. From left to right: Anthocyanin of untreated petal limb (anthocyanin is deposited in the vacuole, resulting in a very even distribution of color), almost complete lack of color in AqANS-silenced petal limb, and punctate pattern of carotenoid deposition in plastids of AqEMF2-silenced petal limb. B. Expression of several A. x coerulea homologs of genes important in carotenoid production (CRTISO and PSY) and degradation (CCD4 and NCED3) in AqANS-silenced control petals (C1-C4) and AqFIE (F1-F6) and AqEMF2 (E1 and E2) treated petals. Petals with strong yellow pigment are highlighted in dark yellow (F1, F5, and E1) and petals with pale yellow pigment are highlighted in light yellow (F2-F4). The expression of these genes is not consistently affected in the AqFIE and AqEMF2 silenced petal samples. It is possible that other genes in the carotenoid pathway are being misexpressed. Scale bars: 10 μm.

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Gleason and Kramer BMC Plant Biology 2013 13:185   doi:10.1186/1471-2229-13-185