Additional file 2.

Heteroblasty in A. x coerulea leaves. A. Unvernalized leaf with 3 major lobes in the lateral leaflets. B. Unvernalized leaf with 2 major lobes in the lateral leaflets. C. Vernalized leaf with higher order petiolules where the central lobe of each leaflet is a separate leaflet borne on a petiolule (asterisks). D. Vernalized leaf with 2 major lobes in the lateral leaflets. These leaves are more deeply lobed than similar unveralization leaves. E. Average number of higher order petiolules within medial or lateral leaflets in wild type unvernalized, AqFIE silenced unvernalized, wild type vernalized, and AqFIE silenced leaves with standard deviations. Both unvernalized and vernalized AqFIE silenced lateral leaflets had on average more higher order petiolules than the wild type. Unvernalized AqFIE silenced lateral leaflets also had a slightly higher average number of higher order petiolules compared to wild type, but vernalized AqFIE silenced leaves had a slightly lower number of petiolules per medial leaflet. When quantified, this increase is significant (*) at pā€‰<ā€‰0.05 for unvernalized lateral leaflets but not significant for the other stages/leaflet types.3

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Gleason and Kramer BMC Plant Biology 2013 13:185   doi:10.1186/1471-2229-13-185