Figure 3.

RT-PCR expression data in PRC2 VIGS-treated leaves.AqIPP2 was used as a loading control for all reactions. Note that the expression of AqFIE and AqEMF2 are low relative to the expression of AqIPP2. A. Expression of AqFIE and AqEMF2 in AqANS-silenced control leaves (C1-C4), and AqFIE-silenced (F1-F8) and AqEMF3-silenced (E1-E4) leaves. AqFIE is clearly down-regulated in AqFIE-silenced tissue and, likewise, AqEMF2 is down-regulated in AqEMF2-silenced tissue. Interestingly, AqEMF2 also appears to be down-regulated in AqFIE-treated leaves but AqFIE expression is unaffected in AqEMF2-treated leaves. B. Expression of several floral organ identity genes in AqANS-silenced control leaves (C1-C4), and AqFIE-silenced (F1-F8) and AqEMF3-silenced (E1-E4) leaves. In several of the AqFIE down-regulated leaves and all of the AqEMF2 down-regulated leaves, AqAG1 is over-expressed compared to AqANS-silenced control leaves. While the expression of the SEPALLATA homologs is variable in both control and experimental leaves, AqSEP3 may be up-regulated in some of the AqFIE- and all of the AqEMF2-silenced leaves. C. Expression of several of the A. x coerulea class I KNOX genes in AqANS-silenced control leaves (C1-C4), and AqFIE-silenced (F1-F8) and AqEMF3-silenced (E1-E4) leaves. Expression of these genes is unaffected in the mature AqFIE- and AqEMF2-silenced leaves.

Gleason and Kramer BMC Plant Biology 2013 13:185   doi:10.1186/1471-2229-13-185
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