Additional file 1.

Infiltration of wounded A. thaliana leaves with calcium channel blockers and calcium chelators abolishes ROS production. Different concentrations of verapamil, EGTA and oxalate were vacuum-infiltrated into detached leaves. Leaves were then wounded and stained with DCF-DA and visualized with a fluorescence microscope (ROS in green and chlorophyll autofluorescence in red). Densitometric analysis of the ROS signal is displayed on the right of each image series. Asterisks represent significant differences using Student's t test relative to water-treated control; *P < 0.05, **P < 0.01.

Format: TIFF Size: 255KB Download file

Beneloujaephajri et al. BMC Plant Biology 2013 13:160   doi:10.1186/1471-2229-13-160