ROS and calcium accumulate in the same cells with different kinetics. (A) Redox potential after wounding of A. thaliana leaves is measured with roGFP2 expressing plants. Upper panels: microscope images display key time points on the time course. Lower panels: kinetics resulting from the quantification of the fluorescent signals. (B) Leaf discs obtained from plants constitutively expressing YC3.6 protein were infiltrated with DCF-DA and gently wounded with a yellow tip. FRET efficiency (upper panel) and ROS formation (lower panel) were scored in a time course with a confocal microscope taking a set of pictures every minute. The experiments were repeated 10 times giving comparable kinetics, one representative time-course is presented.
Beneloujaephajri et al. BMC Plant Biology 2013 13:160 doi:10.1186/1471-2229-13-160