Production of reactive oxygen species and wound-induced resistance in Arabidopsis thaliana against Botrytis cinerea are preceded and depend on a burst of calcium
1 Department of biology, University of Fribourg, Ch. du Musée 10, 1700 Fribourg, Switzerland
2 Department of Biosciences, University of Milan, via G. Celoria 26, 20133 Milan, Italy
3 Current address: Medion Grifols Diagnostics AG, Bonnstrasse 9, 3186 Düdingen, Switzerland
BMC Plant Biology 2013, 13:160 doi:10.1186/1471-2229-13-160Published: 17 October 2013
Additional file 1:
Infiltration of wounded A. thaliana leaves with calcium channel blockers and calcium chelators abolishes ROS production. Different concentrations of verapamil, EGTA and oxalate were vacuum-infiltrated into detached leaves. Leaves were then wounded and stained with DCF-DA and visualized with a fluorescence microscope (ROS in green and chlorophyll autofluorescence in red). Densitometric analysis of the ROS signal is displayed on the right of each image series. Asterisks represent significant differences using Student's t test relative to water-treated control; *P < 0.05, **P < 0.01.
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