Effect of TCTP on BAX induced cell death. Cell death quantification of N. benthamiana leaf discs transiently over-expressing Arabidopsis thaliana TCTP (AtTCTP) and/or BAX. A shows ion leakage calculated relative to time point T0 in response to 2 μM dexamethasone (DEX), which induced expression of BAX. Statistical significances were calculated using analysis of variance (P < 0.05) and are indicated as follows: a BAX vs. AtTCTP, b AtTCTP + BAX vs. AtTCTP, c AtTCTP + BAX vs. BAX. B shows western blot analyses of leaf discs expressing AtTCTP over time (following time points are shown: 2, 21, 27, 45, 54, and 69 hours of disc floating); AtTCTP is shown without (upper panel) and with (lower panel) co-expression of BAX. C shows tobacco leaf discs expressing AtTCTP, BAX, or both while floating on 2 μM DEX containing solution for 69 hours (panel on the left side). Panels on the right side show trypan blue staining of dead cells from leaf discs expressing BAX and/or AtTCTP as indicated. D shows control experiments: relative ion leakage measurements in response to expression of ΔC-BAX, StrepII-tag, or co-expression of ΔC-BAX + AtTCTP in response to 2 μM DEX. No statistically significant differences were calculated using analysis of variance.
Hoepflinger et al. BMC Plant Biology 2013 13:135 doi:10.1186/1471-2229-13-135