Open Access Research article

Perturbation of Brachypodium distachyon CELLULOSE SYNTHASE A4 or 7 results in abnormal cell walls

Pubudu P Handakumbura12, Dominick A Matos13, Karen S Osmont1, Michael J Harrington1, Kyuyoung Heo4, Kabindra Kafle5, Seong H Kim5, Tobias I Baskin1 and Samuel P Hazen1*

Author Affiliations

1 Biology Department, University of Massachusetts, Amherst, MA, USA

2 Plant Biology Graduate Program, University of Massachusetts, Amherst, MA, USA

3 Molecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, MA, USA

4 Department of Polymer Science and Engineering, University of Massachusetts, Amherst, MA, USA

5 Department of Chemical Engineering, Pennsylvania State University, University Park, PA, USA

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BMC Plant Biology 2013, 13:131  doi:10.1186/1471-2229-13-131

Published: 11 September 2013

Additional files

Additional file 1: Figure S1:

Phylogenetic analysis of A. thaliana, B. distachyon and rice CESA superfamily amino acid sequences. A consensus phylogeny was constructed with the neighbor-joining method with 1000 bootstrap permutations. The CESA clade is illustrated as an expanded sub-tree and the CSL clades are illustrated as condensed sub-trees.

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Additional file 2: Figure S2:

Alignment of the B. distachyon three secondary cell wall CESA amino acid sequences. The overhead blue line shows the RING-type zinc finger motif, with cystine residues highlighted in blue. The overhead green lines show the two hypervariable regions. Pink highlights the three putatively catalytic aspartate residues and purple highlights the QxxRW motif. The overhead yellow line shows the plant-specific region and overhead black lines show the eight putative transmembrane domains.

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Additional file 3: Table S1:

The ratios of relative transcripts abundance of the CESAs in root (R), leaf (L) and stem (S) tissue.

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Additional file 4: Figure S3:

Relative expression of selected non-targeted BdCESA genes. Transcript abundance measured by RT-QPCR. The boxes comprise data from three to five individuals from three to four independent transgenic lines. Stem tissue was collected at the same development stage when inflorescence was just emerging from the flag leaf. Box plots and significance are as described for Figure 5.

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Additional file 5: Table S2:

List of oligonucleotides used in this study.

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