Open Access Highly Accessed Research article

Characterization of the basal angiosperm Aristolochia fimbriata: a potential experimental system for genetic studies

Barbara J Bliss12*, Stefan Wanke3, Abdelali Barakat14, Saravanaraj Ayyampalayam5, Norman Wickett16, P Kerr Wall17, Yuannian Jiao1, Lena Landherr1, Paula E Ralph1, Yi Hu1, Christoph Neinhuis3, Jim Leebens-Mack5, Kathiravetpilla Arumuganathan8, Sandra W Clifton109, Siela N Maximova11, Hong Ma11213 and Claude W dePamphilis1*

Author Affiliations

1 Department of Biology, Institute of Molecular Evolutionary Genetics, and the Huck Institutes of the Life Sciences, 201 Life Sciences Building, Pennsylvania State University, University Park, PA 16802, USA

2 USDA ARS PBARC, 64 Nowelo St., Hilo, HI 96720, USA

3 Technische Universität Dresden, Institut für Botanik, D-01062, Dresden, Germany

4 100 Jordan Hall, Clemson University, Clemson, SC, 29634, USA

5 Department of Plant Sciences, University of Georgia, Athens, GA, 30602, USA

6 Chicago Botanic Garden, Glencoe, IL, 27709, USA

7 BASF Plant Science, 26 Davis Drive, Research Triangle Park, NC, 27709, USA

8 Benaroya Research Institute at Virginia Mason, Flow Cytometry and Imaging Core Laboratory, 1201 Ninth Avenue, Seattle, WA, 98101, USA

9 The Genome Institute,Washington University School of Medicine, 4444 Forest Park Boulevard, St. Louis, MO, 63108, USA

10 Stephenson Research and Technology Center, Advanced Center for Genome Technology, University of Oklahoma, 101 David L. Boren Blvd, Norman, OK, 73019, USA

11 Department of Horticulture, 421 Life Sciences Building, Pennsylvania State University, University Park, PA, 16802, USA

12 State Key Laboratory of Genetic Engineering and the Institute of Plant Biology, the Center for Evolutionary Biology, the School of Life Sciences, Fudan University, Shanghai, 200433, China

13 Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China

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BMC Plant Biology 2013, 13:13  doi:10.1186/1471-2229-13-13

Published: 24 January 2013

Additional files

Additional file 1:

Phylogram of Aristolochiaceae relationships. Maximum likelihood analyses showing minimal variation in branch lengths within Aristolochiaceae. Only one maxium likelihood tree was found.

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Additional file 2:

Cultivation Supplement. Description of perianth maturation, hand pollination methods, and self-compatibility in A. fimbriata and A. elegans includes: Figure S1 - Aristolochia fimbriata flower stages. Figure S2 - Hand pollination of A. fimbriata. Table S1 - Evaluating self-compatibility in Aristolochia fimbriata. Table S2 - Fruit set in Aristolochia elegans resulting from hand pollination.

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Additional file 3:

Maximum likelihood analysis of orthologs for alpha-galactosidase (ATAGAL1; AT5G08380). Blue star indicates a gene duplication in a common ancestor of angiosperms.

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Additional file 4:

Maximum likelihood analysis of orthologs for MYB-domain protein ASYMMETRIC LEAVES 1 (AS1; AT2G37630). Blue star indicates a gene duplication in a common ancestor of angiosperms, while the green star indicates a gene duplication in A. fimbriata.

Format: PDF Size: 117KB Download file

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