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Open Access Research article

Characterization of temperature and light effects on the defense response phenotypes associated with the maize Rp1-D21 autoactive resistance gene

Adisu Negeri1, Guan-Feng Wang1, Larissa Benavente2, Cromwell M Kibiti3, Vijay Chaikam3, Guri Johal3 and Peter Balint-Kurti12*

Author Affiliations

1 Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695, USA

2 USDA-ARS, Plant Science Research Unit, North Carolina State University, Raleigh, NC 27695, USA

3 Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA

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BMC Plant Biology 2013, 13:106  doi:10.1186/1471-2229-13-106

Published: 26 July 2013

Abstract

Background

Rp1 is a complex locus of maize, which carries a set of genes controlling race-specific resistance to the common rust fungus, Puccinia sorghi. The resistance response includes the “Hypersensitive response” (HR), a rapid response triggered by a pathogen recognition event that includes localized cell death at the point of pathogen penetration and the induction of pathogenesis associated genes. The Rp1-D21gene is an autoactive allelic variant at the Rp1 locus, causing spontaneous activation of the HR response, in the absence of pathogenesis. Previously we have shown that the severity of the phenotype conferred by Rp1-D21 is highly dependent on genetic background.

Results

In this study we show that the phenotype conferred by Rp1-D21 is highly dependent on temperature, with lower temperatures favoring the expression of the HR lesion phenotype. This temperature effect was observed in all the 14 genetic backgrounds tested. Significant interactions between the temperature effects and genetic background were observed. When plants were grown at temperatures above 30°C, the spontaneous HR phenotype conferred by Rp1-D21 was entirely suppressed. Furthermore, this phenotype could be restored or suppressed by alternately reducing and increasing the temperature appropriately. Light was also required for the expression of this phenotype. By examining the expression of genes associated with the defense response we showed that, at temperatures above 30°C, the Rp1-D21 phenotype was suppressed at both the phenotypic and molecular level.

Conclusions

We have shown that the lesion phenotype conferred by maize autoactive resistance gene Rp1-D21 is temperature sensitive in a reversible manner, that the temperature-sensitivity phenotype interacts with genetic background and that the phenotype is light sensitive. This is the first detailed demonstration of this phenomenon in monocots and also the first demonstration of the interaction of this effect with genetic background. The use of temperature shifts to induce a massive and synchronous HR in plants carrying the Rp1-D21 genes will be valuable in identifying components of the defense response pathway.

Keywords:
Maize; Hypersensitive response; Disease resistance; Temperature sensitive; Light dependent; Rp1; Autoactive R gene