Subcellular localizations of Physcomitrella NDB type proteins in Physcomitrella tissue. GFP was fused to the C-terminus of NDB1, NDB2 and NDB4 from Physcomitrella patens (Pp). Additionally, the last 10 amino acids were fused at the C-terminus of GFP. Subcellular targeting was analyzed in Physcomitrella protonemal tissue along with RFP tagged mitochondrial, peroxisomal or plastid controls. The protein accession number, the location of GFP and the number of amino acids used are shown for each construct. Mitochondria (M), Plastids (Pl) and peroxisomes (Px) are indicated. M-RFP - mitochondrial RFP, Px-RFP - peroxisomal RFP, Pl-RFP - platid RFP. Scale bar indicates 10 μm.
Xu et al. BMC Plant Biology 2013 13:100 doi:10.1186/1471-2229-13-100