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Open Access Highly Accessed Research article

The bHLH transcription factor SPATULA regulates root growth by controlling the size of the root meristem

Srilakshmi Makkena1 and Rebecca S Lamb12*

Author Affiliations

1 Plant Cellular and Molecular Biology Graduate Program, The Ohio State University, Columbus, OH, USA

2 Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA

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BMC Plant Biology 2013, 13:1  doi:10.1186/1471-2229-13-1

Published: 2 January 2013

Additional files

Additional file 1:

SPTis expressed in roots. RT-PCR using total RNA isolated from L. er 7 DAG seedling roots (7 DAG). Two independent biological replicates are shown. SPT product is on the left and ACTIN product is on the right. (A) Sample 1. (B) Sample 2.

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Additional file 2:

spt-11RAMs contain more dividing cells. Micrographs of 5 DAG root tips expressing the G2-M marker CYCB1;1::GUS. (A) Col-0. (B) spt-11.

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Additional file 3:

Vascular cell fate is not altered inspt-11mutants. Micrographs of 5 DAG roots stained with propidium iodine. (A, B) Expression of the xylem-associated pericycle marker J0121::GFP. (C, D) Expression of the companion cell marker CoYMV::GFP. (A, C) Col-0. (B, D) spt-11.

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Additional file 4:

SPTacts additively with GA. Photographs of adult plants. (A) Representative Col-0, spt-11, ga3ox1-2; ga3ox2-1, ga3ox1-2; ga3ox2-1; spt-11/+ and ga3ox1-2; ga3ox2-1; spt-11 plants. (B) Representative L. er, spt-2, ga1-3, ga1-3; spt-2/+ and ga1-3; spt-2 plants.

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Additional file 5:

SPTand GA act additively.

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Additional file 6:

Marker lines used in this study.

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Additional file 7:

Primers used in this study.

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Additional file 8:

Primer combinations used for genotyping.

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Additional file 9:

Primer combinations used in qRT-PCR.

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