Induction of the effector genes in the Arabidopsis and P. sojae interactions. Expression levels of two P. sojae effector genes, PsAvh223 and PsAvh224, highly induced in the soybean-P. sojae interaction were determined in an RT-PCR experiment. Detached leaves of pss1, pen1-1 and Col-0 were inoculated with P. sojae or treated with sterile water droplets. The cDNA samples were used to amplify the two effector genes of P. sojae and Arabidopsis actin gene. Enhanced expression of both effector genes were observed in pss1 but not in pen1-1 and Col-0. 1dC, 1 day post water droplet treatment of detached leaves; 3dC, 3 days post water droplet treatment of detached leaves; 1dT, 1 day post inoculation with P. sojae zoospores; 3dT, 3 day post inoculation with P. sojae zoospores. Actin was used as an internal control.
Sumit et al. BMC Plant Biology 2012 12:87 doi:10.1186/1471-2229-12-87