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Defects in leaf carbohydrate metabolism compromise acclimation to high light and lead to a high chlorophyll fluorescence phenotype in Arabidopsis thaliana

Jessica Schmitz1, Mark Aurel Schöttler2, Stephan Krueger1, Stefan Geimer3, Anja Schneider4, Tatjana Kleine4, Dario Leister4, Kirsten Bell1, Ulf-Ingo Flügge1 and Rainer E Häusler1*

Author Affiliations

1 University of Cologne, Botanical Institute, Biocenter Cologne, Zülpicher Str. 47B, D-50674 Cologne, Germany

2 Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Potsdam-Golm, Germany

3 Universität Bayreuth, Zellbiologie/Elektronenmikroskopie NW I/B1, Universitätsstrasse 30, D-95447 Bayreuth, Germany

4 Biozentrum der Ludwig-Maximilians-Universität München, Department Biologie I - Botanik Großhaderner Str. 2-4, D-82152 Planegg-Martinsried, Germany

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BMC Plant Biology 2012, 12:8  doi:10.1186/1471-2229-12-8

Published: 16 January 2012

Additional files

Additional file 1:

Distribution of 'grana stack number classes' in LL- and HL- grown wild-type and double mutant plants. The number of stacks of 33 to 105 individual grana was determined on TEM images of two to three chloroplasts per line and grouped into classed between 2 and 17 stacks per granum. The number of each class was expressed as percentage of the total number of grana counted. The distribution of grana stack numbers was calculated from (A) 105, (B) 77, (C) 75, and (D) 33 individual grana of LL- and HL-grown Col-0 (A, B) or adg1-1/tpt-2 (C, D).

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Additional file 2:

Protein composition of the >1000 kDa supercomplex. Thylakoid proteins were isolated from wild-type plants by Blue-Native PAGE. The respective protein band was cut out and further analyzed by LC/MS2. The ion score represents -10·Log(P), where P is the probability that an observed match is a random event. Ion scores >38 indicate either identity or extensive homology (P < 0.05). Proteins marked by an asterisk indicate putative new subunits of the NDH Supercomplex (compare [27]).

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Additional file 3:

Light dependency of acceptor- and donor site limitation of PSI determined with HL- and LL-grown wild-type and mutant plants. Quantum efficiencies of acceptor (blue circles, ΦNA) or donor site (red circles, ΦND) limitation of HL- and LL-grown Col-0 wild-type (A, E), adg1-1 (B, F) and tpt-2 (C, G) single mutant as well as the adg1-1/tpt-2 (D, H) double mutant plants obtained from light saturation curves. The data represent the mean ± SE of 12 independent measurements.

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Additional file 4:

Effects of O2 deprivation on Chl-a fluorescence. Chl-a fluorescence traces of HL-grown Col-0 and adg1-1/tpt-2 double mutant plants flushed either with air, i.e. in the presence of 21% O2 (A, B), or with N2, i.e. in the absence of O2 (C, D) in a closed Perspex chamber. Where indicated by arrows, FR illumination was either switched on (+FR) or off (-FR). SP indicates the application of saturated light pulsed at a duration of 0.8 s.

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Additional file 5:

Immunoblots of thylakoid proteins of sucrose-fed wild-type and adg1-1/tpt-2 plants compared to the unfed controls. Immunoblots of photosynthesis associated proteins after separation of 10 μg total protein isolated from HL-grown Col-0 and adg1-1/tpt-2 double mutant on SDS-PAGE. Plants were grown either in the absence (MS) or presence of 50 mM Suc. P*-Threonin indicates signals obtained following incubation of the blots with a phospho-threonin antibody. The numbers indicate signals from PsbC (1), CaS (2), PsbA/PsbD (3), and LhcbII (4).

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Additional file 6:

Phenotypes and Chl-a fluorescence images of wild-type and mutant plants. Description of data: Phenotypic appearance as well as modulated Chl-a fluorescence false color images of the Fv/Fm ratio of the same lines grown in HL or LL for 4 weeks. (A) pgi1-1, pgm1, pgi1-/tpt-2, tpt-2/pgm1, (B) mex1-2, mex1-2/tpt-2, (C) sex1-3, and sex1-3/tpt-2. The color scale indicates the numeric values of the Fv/Fm ratios.

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Additional file 7:

Oligonucleotide primers. Primers used for the identification of T-DNA mutants by PCR or transcript amounts by RT-PCR or qRT-PCR (RL).

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