Table 2

HPLC analysis of leaf carotenoid content (μg/g FW) in dark-adapted plants

Carotenoid content (μg/g FW)


neoxanthin

violaxanthin

antheraxanthin

lutein

zeaxanthin

α-carotene

β-carotene

β-β-xantophylls

xanthophylls/carotenes

WT

18.6 ± 1.6a

44.1 ± 5.8a

nd

105.6 ± 22.4a

nd

4.4 ± 0.6a

62.1 ± 14.8a

62.7 ± 6.0a

2.5 ± 0.7a

lut2

22.1 ± 0.7b

94.7 ± 4.9b

14.6 ± 1.4a

nd

5.0 ± 0.6a

nd

74.9 ± 5.1a

136.4 ± 5.1b

1.8 ± 0.1a

chy1chy2lut5

0.3 ± 0.2c

0.9 ± 0.6c

nd

69.1 ± 4.7b

nd

51.0 ± 2.7b

42.8 ± 1.4b

1.2 ± 0.6c

0.7 ± 0.1b

chy1chy2lut2lut5

8.4 ± 0.1d

13.6 ± 0.2d

8.5 ± 0.1b

nd

3.4 ± 0.1b

nd

109.4 ± 2.3c

33.9 ± 0.3d

0.3 ± 0.1c


Plants grown at 30 μmol photons m-2 s-1 for 6 weeks were dark-adapted, then carotenoids were extracted and quantified via LC-DAD-MS. Data are expressed as mean ± SD (n = 4). nd, not detectable; FW, fresh weight. Values marked with the same letters are not significantly different from each other within a column (P > 0.05).

Fiore et al. BMC Plant Biology 2012 12:50   doi:10.1186/1471-2229-12-50

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