Real-time PCR expression profile of Arabidopsis carotenoid hydroxylase genes. For individual genes, the relative mRNA levels were normalized with respect to the TUBULIN housekeeping transcript and then to wild-type levels. RNA was extracted from dark-adapted, 6-weeks-old plants (see Methods for details). Data are expressed as mean ± SD (n = 3).
Fiore et al. BMC Plant Biology 2012 12:50 doi:10.1186/1471-2229-12-50