Open Access Research article

Perturbation of cytokinin and ethylene-signalling pathways explain the strong rooting phenotype exhibited by Arabidopsis expressing the Schizosaccharomyces pombe mitotic inducer, cdc25

Natasha D Spadafora12, David Parfitt1, Angela Marchbank1, Sherong Li1, Leonardo Bruno2, Rhys Vaughan1, Jeroen Nieuwland1, Vicky Buchanan-Wollaston3, Robert J Herbert4, Maria Beatrice Bitonti2, John Doonan5, Diego Albani6, Els Prinsen7, Dennis Francis1 and Hilary J Rogers1*

Author Affiliations

1 School of Biosciences, Cardiff University, Cardiff CF10 3AT, UK

2 Dipartimento di Ecologia, Università della Calabria, Arcavacata di Rende, Cosenza I-87030, Italy

3 School of Life Sciences, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK

4 Institute of Science and the Environment, University of Worcester, Henwick Grove, Worcester WR2 6AJ, UK

5 Plant Phenomics Centre, Institute of Biological, Environmental and Rural Sciences, Penglais, Aberystwyth University, Ceredigion SY23 3DA, Aberystwyth, UK

6 Department of Botanical, Ecological and Geological Sciences, University of Sassari, Via Piandanna 4, Sassari 07100, Italy

7 Department of Biology, University of Antwerp, Groenenborgerlaan 171, Antwerp B-2020, Belgium

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BMC Plant Biology 2012, 12:45  doi:10.1186/1471-2229-12-45

Published: 27 March 2012

Additional files

Additional file 1:

Expression levels of Spcdc25 in transgenic lines of Arabidopsis. RT-PCR of BTX::Spcdc25 lines 9 and 10 showing expression of the transgene in both lines (lower band is primer dimer).

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Additional file 2:

Figure S2. Null effect of Spcdc25 expression on cell length in mature regions of primary root. DIC/Nomarski images of regions of primary roots exhibiting young lateral root primordia (YLRP) in WT and Spcdc25. Mean (± SE) cortical cell length. WT = 75.14 ± 10.10 Spcdc25 = 56.86 ± 11.61 μm. n = 25. Bar scale = 40 μm.

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Additional file 3:

Expression of Spcdc25 induces increased rooting in cultured hypocotyls at NAA/Kin: 50/100, 50/200, 25/50, 25/200. Hypocotyls of 14 d old plants of (A) wild type (WT) (B) BTX::Spcdc25 (line 9) cultured on two-way concentration gradients of increasing concentrations (ng ml-1) of naphthyl acetic acid (NAA) and kinetin (Kin) for 30 d; representative grids from 3-6 replicate experiments. Cells boxed indicate the NAA/Kin concentrations used for further analysis. Scale bar = 1 mm.

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Additional file 4:

Table showing genes up-or down-regulated by 2-fold or more (Spcdc25/WT).

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Additional file 5:

Real time PCR verification of the microarray results. Above each histogram bar (Spcdc25/WT) is the microarray result (Spcdc25/WT). n = 3.

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Additional file 6:

Table of real time PCR Primers.

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