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Open Access Research article

Perturbation of cytokinin and ethylene-signalling pathways explain the strong rooting phenotype exhibited by Arabidopsis expressing the Schizosaccharomyces pombe mitotic inducer, cdc25

Natasha D Spadafora12, David Parfitt1, Angela Marchbank1, Sherong Li1, Leonardo Bruno2, Rhys Vaughan1, Jeroen Nieuwland1, Vicky Buchanan-Wollaston3, Robert J Herbert4, Maria Beatrice Bitonti2, John Doonan5, Diego Albani6, Els Prinsen7, Dennis Francis1 and Hilary J Rogers1*

Author Affiliations

1 School of Biosciences, Cardiff University, Cardiff CF10 3AT, UK

2 Dipartimento di Ecologia, Università della Calabria, Arcavacata di Rende, Cosenza I-87030, Italy

3 School of Life Sciences, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK

4 Institute of Science and the Environment, University of Worcester, Henwick Grove, Worcester WR2 6AJ, UK

5 Plant Phenomics Centre, Institute of Biological, Environmental and Rural Sciences, Penglais, Aberystwyth University, Ceredigion SY23 3DA, Aberystwyth, UK

6 Department of Botanical, Ecological and Geological Sciences, University of Sassari, Via Piandanna 4, Sassari 07100, Italy

7 Department of Biology, University of Antwerp, Groenenborgerlaan 171, Antwerp B-2020, Belgium

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BMC Plant Biology 2012, 12:45  doi:10.1186/1471-2229-12-45

Published: 27 March 2012

Abstract

Background

Entry into mitosis is regulated by cyclin dependent kinases that in turn are phosphoregulated. In most eukaryotes, phosphoregulation is through WEE1 kinase and CDC25 phosphatase. In higher plants a homologous CDC25 gene is unconfirmed and hence the mitotic inducer Schizosaccharomyces pombe (Sp) cdc25 has been used as a tool in transgenic plants to probe cell cycle function. Expression of Spcdc25 in tobacco BY-2 cells accelerates entry into mitosis and depletes cytokinins; in whole plants it stimulates lateral root production. Here we show, for the first time, that alterations to cytokinin and ethylene signaling explain the rooting phenotype elicited by Spcdc25 expression in Arabidopsis.

Results

Expressing Spcdc25 in Arabidopsis results in increased formation of lateral and adventitious roots, a reduction of primary root width and more isodiametric cells in the root apical meristem (RAM) compared with wild type. Furthermore it stimulates root morphogenesis from hypocotyls when cultured on two way grids of increasing auxin and cytokinin concentrations. Microarray analysis of seedling roots expressing Spcdc25 reveals that expression of 167 genes is changed by > 2-fold. As well as genes related to stress responses and defence, these include 19 genes related to transcriptional regulation and signaling. Amongst these was the up-regulation of genes associated with ethylene synthesis and signaling. Seedlings expressing Spcdc25 produced 2-fold more ethylene than WT and exhibited a significant reduction in hypocotyl length both in darkness or when exposed to 10 ppm ethylene. Furthermore in Spcdc25 expressing plants, the cytokinin receptor AHK3 was down-regulated, and endogenous levels of iPA were reduced whereas endogeous IAA concentrations in the roots increased.

Conclusions

We suggest that the reduction in root width and change to a more isodiametric cell phenotype in the RAM in Spcdc25 expressing plants is a response to ethylene over-production. The increased rooting phenotype in Spcdc25 expressing plants is due to an increase in the ratio of endogenous auxin to cytokinin that is known to stimulate an increased rate of lateral root production. Overall, our data reveal important cross talk between cell division and plant growth regulators leading to developmental changes.