Relative LPCAT activity in protein fractions from mid-developing siliques containing WT and AS11 embryos at stages 7-8 (as designated in Methods"). LPCAT activity was monitored by appearance of (A) 3 H and (B) 14 C label in PC, during a 20 min incubation of WT or AS11 protein in the presence of L-α-palmitoyl- [1-3 H methyl] lyso-3-phosphatidylcholine and [1-14 C] 18:1-CoA. The data represent the average of two experimental replicates ± S.D. While absolute values differed, similar trends were observed in a second independent biological replication.
Xu et al. BMC Plant Biology 2012 12:4 doi:10.1186/1471-2229-12-4