Figure 6.

Relative LPCAT activity in protein fractions from mid-developing siliques containing WT and AS11 embryos at stages 7-8 (as designated in Methods"). LPCAT activity was monitored by appearance of (A) ³ H and (B) ¹⁴ C label in PC, during a 20 min incubation of WT or AS11 protein in the presence of L-α-palmitoyl- [1-³ H methyl] lyso-3-phosphatidylcholine and [1-¹⁴ C] 18:1-CoA. The data represent the average of two experimental replicates ± S.D. While absolute values differed, similar trends were observed in a second independent biological replication.