BiP induction/stabilization during Zera-DsRed expression. a) immunoblot incubated with anti-R8 antibody (dilution 1:8000). The gel was loaded with 5 μg of total proteins extracted from untransformed tobacco leaves (lane 1), Zera-DsRed transformed leaves after two days post infiltration (lane2), four days post-infiltration (lane 3) six days after post-infiltration (lane 4) and ten days post infiltration (lane 5). Arrow indicates Zera-DsRed protein, arrowheads indicated oligomers of Zera-DsRed reactive to R8 antibody. b) Immunoblot incubated with anti-BiP antibody (dilution 1:2000). The gel was loaded with 5 μg of total proteins extracted as in a). Arrow indicates the presence of BiP protein in protein extracts.
Joseph et al. BMC Plant Biology 2012 12:36 doi:10.1186/1471-2229-12-36