Open Access Research article

Transcriptional profile analysis of E3 ligase and hormone-related genes expressed during wheat grain development

Delphine Capron1, Said Mouzeyar1, Aurélia Boulaflous1, Christine Girousse2, Camille Rustenholz2, Christel Laugier2, Etienne Paux2 and Mohamed Fouad Bouzidi1*

Author Affiliations

1 Université Blaise Pascal, UMR 1095 GDEC, 24 avenue des Landais, F-63177 Aubière, France

2 INRA, UMR 1095 GDEC, 234 avenue du Brézet, F-63100 Clermont-Ferrand, France

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BMC Plant Biology 2012, 12:35  doi:10.1186/1471-2229-12-35

Published: 14 March 2012

Additional files

Additional file 1:

Normalized data from wheat grain development microarray expression analysis. Data are presented as median values.

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Additional file 2:

Information of enriched GO. GOID, term GO, process, p-value and probesets were listed in the file.

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Additional file 3:

Number of common sequences (the whole set of DEGs, E3 ligase and Hormone-related genes) identified by sequence similarity between Affymetrix wheat targets (Wan et al., 2008)/EST (Laudencia-Chingcuanco et al., 2006) and the UniGene sequences identified in this study.

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Additional file 4:

Putative tissue location of the transcripts DEGs.

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Additional file 5:

Details of Blast2GO analysis.

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Additional file 6:

Details of E3 ligase genes.

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Additional file 7:

The relative distribution of the E3 ligase and hormone-related genes between cluster 1 (up-regulated genes) and cluster 2 (down-regulated genes).

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Additional file 8:

Details of hormone-related genes.

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Additional file 9:

Validation of array data by comparing expression estimates obtained from NimbleGen wheat microarray and quantitative reverse transcription PCR for differentially expressed E3 ligase and hormone-related genes. (A) -(H): E3 ligase genes. (I) -(K): Hormone-related genes. Blue graphs represent DNA microarray data depicting the expression intensity of each transcript (left y-axis); red graphs depict quantitative reverse transcription RT-PCR results (right y-axis representing the relative level of expression) at 11 developmental stages (x-axis representing the thermal time after anthesis (°Cdays)). The correlation coefficient (R) between the two graphs is indicated for each gene.

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Additional file 10:

Primer sequences used for qRT-PCR analysis.

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