Open Access Research article

Some ethylene biosynthesis and AP2/ERF genes reveal a specific pattern of expression during somatic embryogenesis in Hevea brasiliensis

Piyanuch Piyatrakul12, Riza-Arief Putranto13, Florence Martin1, Maryannick Rio1, Florence Dessailly1, Julie Leclercq1, Jean-François Dufayard1, Ludovic Lardet1 and Pascal Montoro1*

Author Affiliations

1 CIRAD, UMR AGAP, F-34398, Montpellier, France

2 Rubber Research Institute, Chatuchak, Bangkok, 10900, Thailand

3 Indonesian Biotechnology Research Institute for Estate Crops, Bogor, Indonesia

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BMC Plant Biology 2012, 12:244  doi:10.1186/1471-2229-12-244

Published: 26 December 2012

Additional files

Additional file 1: Figure S1:

Phylogenetic tree of the AP2 family. The deduced amino acid sequences of the AP2 domain from Hevea (black letter) and Arabidopsis (blue letter) were aligned using Muscle, and the phylogenetic tree was constructed using PhyML with an LG+T model. Hevea somatic embryogenesis marker genes are indicated in bold letters.

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Additional file 2: Figure S2:

Phylogenetic tree of ERF group I. The deduced amino acid sequences of the AP2 domain from Hevea (black letter) and Arabidopsis (blue letter) were aligned using Muscle, and the phylogenetic tree was constructed using PhyML with an LG+T model. Hevea somatic embryogenesis marker genes are indicated in bold letters.

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Additional file 3: Figure S3:

Phylogenetic tree of ERF group III. The deduced amino acid sequences of the AP2 domain from Hevea (black letter) and Arabidopsis (blue letter) were aligned using Muscle, and the phylogenetic tree was constructed using PhyML with an LG+T model. Hevea somatic embryogenesis marker genes are indicated in bold letters.

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Additional file 4: Figure S4:

Phylogenetic tree of ERF group IV. The deduced amino acid sequences of the AP2 domain from Hevea (black letter) and Arabidopsis (blue letter) were aligned using Muscle, and the phylogenetic tree was constructed using PhyML with an LG+T model. Hevea somatic embryogenesis marker genes are indicated in bold letters.

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Additional file 5: Figure S5:

Phylogenetic tree of ERF group VI. The deduced amino acid sequences of the AP2 domain from Hevea (black letter) and Arabidopsis (blue letter) were aligned using Muscle, and the phylogenetic tree was constructed using PhyML with an LG+T model. Hevea somatic embryogenesis marker genes are indicated in bold letters.

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Additional file 6: Figure S6:

Phylogenetic tree of ERF group X. The deduced amino acid sequences of the AP2 domain from Hevea (black letter) and Arabidopsis (blue letter) were aligned using Muscle, and the phylogenetic tree was constructed using PhyML with an LG+T model. Hevea somatic embryogenesis marker genes are indicated in bold letters.

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Additional file 7: Table S1:

List of primer sequences for 132 AP2/ERF genes from H. brasiliensis clone PB 260.

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Additional file 8: Table S2:

Comparison Cp value of callus with various embryogenic capacities grown on embryogenesis induction medium (ENT) and somatic embryogenesis expression medium (EXP){Calli are from three types of lines: (NE) non-embryogenic line (CI04115), (E) embryogenic and non-regenerant line (CI04106), and (R) regenerant line (CI07060)}, normal and abnormal embryo and 3 organ of plantlet:(L) Leaf, (S) Stem, (R) Root in 11 house keeping genes.

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