Table 2 |
|||||
| Tomato orthologues of candidate regulatory genes for L-ascorbic acid metabolism | |||||
| Gene | Reference Gene inArabidopsis | Unigene | Name | F-primer (5′-3′) | R-primer (5′-3′) |
| L-galactose pathway | |||||
| GDP-mannose pyrophosphorylase | At2g39770 | SGN-U568548 | SlGMP1 | CAGGTAGCATTATCGGTTGG | TTGATCTCCTTGTGGGGTAA |
| SGN-U568547 | SlGMP2 | GTGTAGTTTTGCCCCACAAG | AGGAGAACTGGAAACCAACC | ||
| SGN-U584300 | SlGMP3 | AGAAGCGACTGGGAGAGTTG | GAATCCGGTCCAAAACAGAA | ||
| GDP-mannose-3,5-epimerase | At5g28840 | SGN-U581327 | SlGME1 | TTCCTGTCCAACACATTCCT | CTTTCTCGATCTGCTCCTTG |
| SGN-U580362 | SlGME2 | ATTCGAGATGTGGGGAGATG | GGAAGGTTCTTGCCATCAAA | ||
| GDP-L-galactose phosphorylase | At4g26850 | SGN-U579800 | SlGGP1 | TCCAAGTGTCGTTGCCATAA | TCGGAAGTAAGGGTTTGCTG |
| SGN-U573852 | SlGGP2 | GCTCTCCATTTTGCCAGAGA | CCCCTTCCTTGCCAGTATTT | ||
| L-galactose-1-P-phosphatase | At3g02870 | SGN-U600217 | SlGPP1 | TGGTGGCGCTGTAATAGTGA | AACGCTTCCTTGAGATGAGG |
| SGN-U568299 | SlGPP2 | AGGTCCCTTCGTATGTGTGG | GAACCCTCCAGCTTCTTTCA | ||
| L-galactose dehydrogenase | At4g33670 | SGN-U565558 | SlGalDH | AGTAGCAACGACTGGAATGG | TGCACAACAGGAGATCACAA |
| L-galactonolactone dehydrogenase | At3g47930 | SGN-U585649 | SlGLDH | AGATTGAGGTTCCCAAGGAC | TTAGATAGGATGCGGTTTGG |
| AsA recycling pathway | |||||
| Monodehydroascorbate reductase | At1g63940 | SGN-U583672 | SlMDHAR1 | CAAGGGTTTCGGTTCCTTCT | CTGCATTTCCTCCTCCAACT |
| At3g52880 | SGN-U573751 | SlMDHAR2 | AGATCGTTGGTGCATTCCTC | AAAACTGATGCCCTCCTGTG | |
| At3g27820 | SGN-U584073 | SlMDHAR3 | AGGAATGGAATGTGCTGCTT | GACCGTGCCTTTCACAAACT | |
| Dehydroascorbate reductase | At1g19570 | SGN-U583361 | SlDHAR1 | AGGTGGCTCTTGGACACTTC | CTTCAGCCTTGGTTTTCTGG |
| At5g16710 | SGN-U577467 | SlDHAR2 | CACCCAGAGGGTTTTGCTTA | CTCCAGTGCCTGTGAGATGA | |
Details of the gene ID, the corresponding unigene, and the forward and reverse primers used in gene expression studies throughout ripening are shown.
Mellidou et al. BMC Plant Biology 2012 12:239 doi:10.1186/1471-2229-12-239
