Table 4 |
|
| Sequence information for primers used for polymorphism and BnFLC.A10 gene expression analysis | |
| Primer name | Sequence(5'-3') |
| BnFLC.A10 specific primers | |
| Exon1-2 f | CATCCGTCGCTCTTCTTGTC |
| Exon1-2 r | GTTGCTTTCCATATCGATCAAG |
| Exon2-4 f | AACATGATGATGATCTTAAAGCCT |
| Exon2-4 r | CTCCAGCTGAACCAGGGAAC |
| Exon4-7 f | CTTGAGGAATCAAATGTCGATAA |
| Exon4-7 r | CGGAGATTTGTCCTGGTGAG |
| InDel1 f (P4 f) | GGTTCCTTTTCTTTTCGTTTGGG |
| InDel1 r (P4 r) | GAAGTAAAGTCGGACAAGAAGG |
| InDel2 f (P5 f) | CCTTCTTGTCCGACTTTACTTC |
| InDel2 r (P5 r) | CGTTGCTCCTACTTTGTCTATC |
| InDel3 (IP1IP2) f | CGTCGCTCTTCTTGTCGTCTC |
| InDel3 (IP1IP2) r | TATGCATCACAGCGTGTCAAA |
| InDel4 f | GTGTTCAGCTGTCGCTTCTAT |
| InDel4 r | CTAACGCTGGCTTTGATCTT |
| Itr1f | AATACTTCCTGCGAATCTTGTG |
| Itr1r | AGTTTGCTTCTAAGTCCCCAAT |
| SSR primers developed from JBnB75D10 | |
| 25GTTA f | ACTTTCATCACCATTGCAGACA |
| 25GTTA r | AAGAGCAGCCATTGTATCAGGT |
| T11 f | TTCCCAAGCTTGCTGGTACT |
| T11 r | GAGATTTCCCTCGCTTGATG |
| NIAB009 f | TACGCTAGTGAGAACACCTCCA |
| NIAB009 r | GCTTTAGCAAGAAAACTCGGAA |
| q-RT PCR primers | |
| Prt f | TCCGTCGCTCTTCTTGTCGT |
| Prt r | GCTGAACCAGGGAACCCACA |
| actin2 F | CTGTGCCAATCTACGAGGGTTTC |
| actin2 R | CTTACAATTTCCCGCTCTGCTGT |
| 18S f | GAGTATGGTCGCAAGGCTGAAA |
| 18S r | CGCTCCACCAACTAAGAACGG |
Hou et al. BMC Plant Biology 2012 12:238 doi:10.1186/1471-2229-12-238
