Cloning of qFT10-4 and detailed structure and allelic divergence of BnFLC.A10. (A) Positions of markers used to fine-map qFT10-4 are shown in the BAC clone JBnB75D10 of B. napus ‘Tapidor’. Marker IP1IP2 was developed from a specific sequence of BnFLC.A10. (B) Genotypes of recombinants detected among non-flowering plants of the BC5F2 segregation population derived from the TN DH line DH043 (winter-type) and Ningyou7 (semi-winter-type). T and H represent homozygous and heterozygous genotypes, respectively, for the Tapidor allele. (C) Genes identified in the 80-kb region of JBnB75D10 that was delimited with markers T11 and Niab009. Arrows show the relative positions of predicted open reading frames (ORFs). For each ORF, the orthologous gene in A. thaliana is marked and genes that lacked an ortholog are labelled ‘hypothetical’. (D) Schematic diagram of the DNA sequence of BnFLC.A10. The arrow shows the translation start site. Roman numerals indicate the indels (I–IV) between the alleles from Tapidor and Ningyou7. Vertical bars labeled with Arabic numerals represent SNPs (1–8). For the SNPs, the nucleotide found in the Tapidor allele is given first. (E) BnFLC.A10 expression as detected by quantitative PCR during different stages of vernalization (0 to 7 weeks) at 4°C. Expression of the Ningyou7 allele decreased much more rapidly than that of the Tapidor allele during vernalization.
Hou et al. BMC Plant Biology 2012 12:238 doi:10.1186/1471-2229-12-238