Additional file 2.

Figure S2. In vitro germination assays of pollen from p5cs1 single mutants. To evaluate possible defects in pollen viability of a mutant bearing a single mutation in P5CS1 gene, pollen from a homozygous knockout p5cs1 mutant was incubated in vitro on germination medium and scored for germination. (A) Percentage of germinated versus total pollens (germinated + non germinated), including both large and small pollen grains (right column), compared to wild type (left column). (B) Percentage of large (middle column), small (right column), and wild type pollen (left column) versus large (middle column), small (right column) and wild type (left column) total germinated pollen. Values in (A) and (B) represent the means of four independent experiments ± SE.

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Mattioli et al. BMC Plant Biology 2012 12:236   doi:10.1186/1471-2229-12-236