Phenotypical analysis of inoculated MtN5hp roots and FLOT4 expression. A. Number of root hair curling events, total and invaded primordia and mature nodules in MtN5hp roots inoculated with S. meliloti. The data reported are means ± SE (n=40) calculated as percentage relatively to control inoculated roots. Student’s t test was applied. ***, P < 0.001. B. The expression level of FLOT4 was assessed by qRT-PCR in adventitious hairy roots obtained by genetic transformation with A. rhizogenes harbouring a pRedRoot binary vector carrying either the MtN5hp construct or an empty T-DNA (control). The expression data were normalized to an internal actin control. The relative expression ratios were calculated using control mock-inoculated roots as calibrator sample. The values reported are means ± SE (n=3). Student’s t test was applied. *, P < 0.05; **, P < 0.01.
Pii et al. BMC Plant Biology 2012 12:233 doi:10.1186/1471-2229-12-233