Effect of specific inhibitors of PLC and PLD (neomycin and n-butanol, respectively) on MtN5 expression. A. The relative mRNA level of MtN5 was assessed by qRT-PCR analysis in wild-type M. truncatula roots either untreated or treated with PLC agonist neomycin and inoculated with S. meliloti. B. qRT-PCR performed on wild-type M. truncatula roots either untreated or treated with PLD agonist n-butanol and inoculated with S. meliloti. C. qRT-PCR carried out on wild-type M. truncatula roots either untreated or treated with n-butanol isomer, t-butanol, and inoculated with S. meliloti. The expression data were normalized to an internal actin control. The relative expression ratios were calculated using untreated, mock-inoculated roots as calibrator sample. The data reported are means ± SE (n = at least 3). Common letters indicate no significant difference according to one-way ANOVA with the Bonferroni post-test.
Pii et al. BMC Plant Biology 2012 12:233 doi:10.1186/1471-2229-12-233