Demonstration of GM077 as a waxy mutant using the corresponding wild type BP034 as a control. (A) Phenotypic traits of BP034 and GM077 grains. The grains, with and without hull (a and b, respectively), are visualized. Transverse sections of the grains without and with iodine-staining were photographed (c and d, respectively). Scale bars (=1.5 mm) are indicated. (B) Content of total starch and amylose was determined as Sun et al. . 1No significant difference of total starch content between BP034 and GM077 (P = 0.425). 2Significant difference of total amylose content between BP034 and GM077 (P = 0.0001). (C) qPCR analysis of expression levels for GBSSI and GBSSII. DW (dry weight), GBSS (granule-bound starch synthase). The statistical difference between BP034 and GM077 is presented as “significantly decreased” (**P < 0.01) and “increased” (*P < 0.05), respectively.
Zhang et al. BMC Plant Biology 2012 12:230 doi:10.1186/1471-2229-12-230