Tomato members of the LRRII and PERK subfamilies interact with NSP of ToYSV. Yeast two-hybrid assay using the kinase domain of LRRII and PERK subfamily members of tomato as bait and the NSP of ToYSV as prey. All co-transformed yeast strains were grown on synthetic defined (SD) medium lacking leucine and tryptophan (SD-leu, -trp), indicating the presence of both plasmids constructs in their cells. Yeast growth on SD medium lacking leucine, tryptophan and histidine (SD -leu, -trp, -his) indicates an interaction between the bait and prey constructs. This was observed in the yeast strains co-expressing NSP and SlNIK1, SlNIK2, SlNIK3, SlBAK1 or SlNsAK. No interaction between NSP and SlEFR was observed. All negative controls using empty vector failed to grow on SD -leu, -trp, -his, indicating the absence of transactivation.
Sakamoto et al. BMC Plant Biology 2012 12:229 doi:10.1186/1471-2229-12-229