LC/MS-analysis of products formed from 9-HPOD by incubation with PpAOS1 and PpAOS2, respectively. (A) Shown is an extracted ion chromatogram (m/z 311) of the RP-HPLC/MS-analysis of products derived from incubation of 9-HPOD with reaction buffer (control), PpAOS1 and PpAOS2. (B) The tandem-MS spectrum of the major peak shown in (A), which is in accordance to those reported earlier for α-ketol derivatives [34,35].
Scholz et al. BMC Plant Biology 2012 12:228 doi:10.1186/1471-2229-12-228