Table 2

Characterization of candidate loci identified through mapping of a “reduced sensitivity to sulfamethoxazole” (RSS) phenotype
Locus Mutations Identified by Mapping T-DNA Lines for Phenotypic Confirmation
Nucleotide Changea Strand Genome Positionb Amino Acid Change Line(s) Examined Phenotype on 3 μM Sulfamethoxazolec
At5g37160 G → A + 14705775 V90M SALK_045992C, SALK_129697C Bleached
At5g37830 C → T - 15059441 S362L SALK_078745C Green
At5g39040 G → A + 15629182 G529E SALK_011884 Bleached
Wildtype n/a n/a n/a n/a n/a Bleached
RSS 26-1 n/a n/a n/a n/a n/a Green

aCandidate loci were identified as described in Methods. The nucleotide change is shown in the context of the coding sequence, independent of strand orientation.

bGenome position as defined by the Arabidopsis Information Resource.

cSeedling phenotypes were evaluated after 14 days of growth on media containing 3 μM sulfamethoxazole. “Bleached”/”Green” describe the appearance of the majority of seedlings for each genotype and represent assessments from at least two independent experiments.

n/a = not applicable.

Schreiber et al.

Schreiber et al. BMC Plant Biology 2012 12:226   doi:10.1186/1471-2229-12-226

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