Figure 3.

Characterization of a “reduced sensitivity to sulfamethoxazole (Smex)” mutant (RSS 26–1). (a) Seedling growth phenotypes of RSS 26–1 and an oxoprolinase1 T-DNA insertion line (oxp1-1) on media containing 3 μM Smex. Images were captured after 14 days of growth. Scale bar represents 1 cm. (b) Gene model of At5g37830 (encoding OXOPROLINASE1). Black regions indicate open reading frames while white areas designate untranslated regions. The location of the point mutation mapped in this study for RSS 26–1 is indicated by an asterisk, and the specific sequence change is shown at both the nucleotide (nt) and amino acid (aa) levels. An inverted triangle denotes the approximate location of the T-DNA insertion (SALK_078745C, oxp1-1) used to confirm the RSS phenotype.