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Open Access Research article

miRNA164-directed cleavage of ZmNAC1 confers lateral root development in maize (Zea mays L.)

Jing Li12, Guanghui Guo12, Weiwei Guo12, Ganggang Guo12, Dan Tong12, Zhongfu Ni12, Qixin Sun12 and Yingyin Yao123*

Author Affiliations

1 State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China

2 National Plant Gene Research Centre (Beijing), Beijing, 100193, China

3 Department of Plant Genetics & Breeding, China Agricultural University, Yuanmingyuan Xi Road No. 2, Haidian District, Beijing, 100193, China

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BMC Plant Biology 2012, 12:220  doi:10.1186/1471-2229-12-220

Published: 21 November 2012

Additional files

Additional file 1:

Polygenetic tree of 175 maize NAC and 105ArabidopsisNAC proteins. The unrooted phylogenetic tree of NAC proteins was constructed using the CLUSTAL X program with the neighbor-joining method. In this figure, 105 Arabidopsis NAC proteins and 175 maize NAC proteins are classified into three large groups, separated by blue lines. Red arcs represent 14 subgroups and pink dots denote putative miR164 target genes in maize. The sequences of 105 Arabidopsis NAC proteins are from reference [3].

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Open Data

Additional file 2:

Putative ZmNACs were obtained as the putative miR164 target genes. This figure shows the reverse complementary site for mature miR164 and 7 ZmNACs.

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Open Data

Additional file 3:

The full-length cDNA of ZmNAC1. This figure shows the full length cDNA of ZmNAC1, in which the highly conserved region of the NAC domain is boxed; the red box indicates the (NLS) nuclear localization signal as noted between amino acids 121 and 138.

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Open Data

Additional file 4:

The figure shows the variation of lateral root density among RILs. 40 RILs were investigated 8days after germination, 4 plants per genotype were investigated for lateral root density. Lateral root numbers in primary root were counted manually and was divided by the total length of the primary root as a parameter for lateral root density (De Smet et al. 2012). Analysis of variance for the lateral root density among 40 RILs were performed.

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Open Data

Additional file 5:

The expression levels ofZmNAC1among RILs. This figure shows the expression of ZmNAC1 in the RILs, which was investigated 8 days after germination by planting eight plants for each genotype, half of which were collected for gene expression analysis.

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Open Data

Additional file 6:

The secondary structure of ZmmiR164b in 87-1 and Zong3. This figure shows the secondary structure of ZmmiR164b in 87-1 and Zong3 as determined by mFold software. The good hairpin structure indicates that both of them can give rise to mature miR164.

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Open Data

Additional file 7:

The correlation between lateral root numbers and expression of mir164b in 40 RILs. This figure shows that a large variation exists in both lateral root numbers and the expression of miR164b among 40 RILs. The correlation coefficient value was calculated, and no significant correlation was found.

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Open Data

Additional file 8:

Comparison of theZmNAC1promoter between 87-1 and Zong3. This figure shows that no large variation exists in the 1 kB region of the ZmNAC1 promoter between two inbred lines, with the exception of one SNP.

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