Effects of mutations in POS9 on promoter activity. Diagrams of the entire INO promoter, promoter deletion and four site-directed mutagenesis constructs are shown. Positions in the diagrams represent the number of base pairs upstream of the putative translational start codon of INO. Ovule specific INO-like GUS activity indicates the number of independent plants exhibiting the outer integument specific GUS staining pattern shown by the full-length P-INO relative to the total number of transformants evaluated, and percentage calculated from these values. The frequency of INO-like expression for pRJM209 was previously described , and was similar to results obtained in the experimental repetition reported here.
Simon et al. BMC Plant Biology 2012 12:214 doi:10.1186/1471-2229-12-214