Ovule expression from combinations of POS9 and truncated forms of POSY. Diagrams of the entire INO promoter and POSY deletion constructs are shown at left. Positions in the diagrams represent the number of base pairs upstream of the putative INO translational start codon. The orange box represents a 60 bp minimal promoter element from the CaMV 35S promoter. Ovule specific INO-like GUS activity indicates the number of independent plants exhibiting the outer integument specific GUS staining pattern shown by the full-length P-INO relative to the total number of transformants evaluated, and percentage calculated from these values. The frequency of INO-like of expression for pRJM77 was previously described , and was similar to that obtained in the experimental repetitions reported here. *Results obtained when a 50X elevated concentration of X-gluc substrate was used.
Simon et al. BMC Plant Biology 2012 12:214 doi:10.1186/1471-2229-12-214