Figure 1.

npr1 alleles differ in their response to MeJA. Forty-three npr1 alleles were treated with 100 μM methyl jasmonate (MeJA), with 0.1% DMSO and 0.02% Silwet L-77 or a mock treatment. One day later, Pseudomonas syringae pv. tomato DC3000 (Pto) was inoculated and its growth measured three days later in a logarithmic scale. For each genotype and treatment three samples with 5 plants per sample were taken. The bars show the average ± SD and npr1-1 and npr1-3 are included as controls for negative and positive response to MeJA respectively. (a) npr1 alleles that show resistance induced by MeJA. (b) npr1 alleles that do not show resistance induced by MeJA. (c) Schematic representation of the npr1 alleles that showed resistance induced by MeJA along the structure of NPR1. BTB/POZ stands for
    B
road-Complex,
    T
ramtrack and
    B
ric-a-brac proteins,
    Po
x virus and
    Z
inc finger proteins. Ankyrin for Ankyrin Repeat Motifs (4 of them) and NLS for Nuclear Localization Signal. The arrows indicate point mutations, the asterisks stop codons, the letter “F” frameshift, and a triangle an internal deletion. The number indicates the number of allele. In all figures, the experiments were repeated at least three times with similar results. One asterisk means a significant difference with P<0.05, and two asterisks means P<0.01 (Student’s test of one tail).

Canet et al. BMC Plant Biology 2012 12:199   doi:10.1186/1471-2229-12-199
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