Open Access Research article

Two putative-aquaporin genes are differentially expressed during arbuscular mycorrhizal symbiosis in Lotus japonicus

Marco Giovannetti1, Raffaella Balestrini1, Veronica Volpe1, Mike Guether14, Daniel Straub2, Alex Costa3, Uwe Ludewig2 and Paola Bonfante1*

Author Affiliations

1 Department of Life Sciences and Systems Biology, University of Torino and IPP-CNR, Viale Mattioli 25, Torino, 10125, Italy

2 Institute of Crop Science, University of Hohenheim, Fruwirthstrasse 20, Stuttgart, 70599, Germany

3 Department of Life Sciences, University of Milano, Via Celoria 26, Milano, 20133, Italy

4 Botanical Institute, Karlsruhe Institute of Technology, Hertzstrasse 16, Karlsruhe, D-76187, Germany

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BMC Plant Biology 2012, 12:186  doi:10.1186/1471-2229-12-186

Published: 9 October 2012

Additional files

Additional file 1:

Aminoacidic sequence alignment of LjNIP1 and LjXIP1. Sequence alignments of LjNIP1 and LjXIP1 with the closest protein found with Blast. The amino acid sequences were compared using CLUSTALW software. The two ‘NPA motifs’ are coloured in light grey. The four residues (R1–R4) forming the selectivity filter are shaded in dark grey.

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Additional file 2:

Prediction of transmembrane helices in LjNIP1 and LjXIP1. The TMrpres2D web tool (http://bioinformatics.biol.uoa.gr/TMRPres2D/) allows us to confirm the putative 6 transmembrane domains, which are typical of the aquaporin class.

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