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Open Access Research article

Grape berry ripening delay induced by a pre-véraison NAA treatment is paralleled by a shift in the expression pattern of auxin- and ethylene-related genes

Fiorenza Ziliotto1, Massimiliano Corso1, Fabio Massimo Rizzini1, Angela Rasori1, Alessandro Botton1 and Claudio Bonghi12*

Author Affiliations

1 Department of Agronomy, Food, Natural resources, Animals and Environment, DAFNAE, University of Padova, Agripolis – Viale dell’Università 16, 35020, Legnaro, Padova, Italy

2 Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia, CIRVE, University of Padova, Agripolis – Viale dell’Università 16, 35020, Legnaro, Padova, Italy

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BMC Plant Biology 2012, 12:185  doi:10.1186/1471-2229-12-185

Published: 9 October 2012

Abstract

Background

Auxins act as repressors of ripening inception in grape (véraison), while ethylene and abscisic acid (ABA) play a positive role as inducers of the syndrome. Despite the increasing amount of information made available on this topic, the complex network of interactions among these hormones remains elusive. In order to shed light on these aspects, a holistic approach was adopted to evaluate, at the transcriptomic level, the crosstalk between hormones in grape berries, whose ripening progression was delayed by applying naphtalenacetic acid (NAA) one week before véraison.

Results

The NAA treatment caused significant changes in the transcription rate of about 1,500 genes, indicating that auxin delayed grape berry ripening also at the transcriptional level, along with the recovery of a steady state of its intracellular concentration. Hormone indices analysis carried out with the HORMONOMETER tool suggests that biologically active concentrations of auxins were achieved throughout a homeostatic recovery. This occurred within 7 days after the treatment, during which the physiological response was mainly unspecific and due to a likely pharmacological effect of NAA. This hypothesis is strongly supported by the up-regulation of genes involved in auxin conjugation (GH3-like) and action (IAA4- and IAA31-like). A strong antagonistic effect between auxin and ethylene was also observed, along with a substantial ‘synergism’ between auxins and ABA, although to a lesser extent.

Conclusions

This study suggests that, in presence of altered levels of auxins, the crosstalk between hormones involves diverse mechanisms, acting at both the hormone response and biosynthesis levels, creating a complex response network.

Keywords:
Vitis vinifera; Hormones; Cross-talk; Fruit development; Microarray; HORMONOMETER; Transcriptomics